Antifibrotics and Antioxidants of Chlorogenic Acid (CGA) Inhibits Toll- Like Receptors (TLR-4) as a Marker of Liver Fibrotic
DOI:
https://doi.org/10.33096/gmj.v3i2.87Kata Kunci:
CCL4 antifibrotic, antioxidant, TLR-4;, Liver, CGAAbstrak
Introduction: Chlorogenic Acid (CGA) is an antifibrotic and antioxidant for fibrotic tissues. These double roles be able to inhibit or fibrotic tissues chains because of internal and external issues. For example, virus, bacteria or other pathogens and also by drugs, alcohol, cigarettes, etc. as external factor that affect quality of body tissues. Toll-Like Receptor-4 (TLR-4) as a marker fibrotic tissues. It is a key for researcher could be find out by expression performance. The aim of this study is to reveal the CGA as a candidate of antifibrotic & antioxidant in liver fibrosis induced by CCL4.
Methods: This is pure experimental research with a simple experimental design or post-test only control group design. A total 29 mices of 2.5-month-old male Swiss mice with weigh 35-40 grams divided into 6 groups: 3 groups of controls (injected by natrium chloride, CGA, and CCL4) and 3 groups of treated (injected by CGA doses 42 mg/kg, 63 mg/kg or 84 mg/kg). The liver organ was used to examine the expression of TLR-4 by rtPCR. This research revealed that expression of TLR-4 lower than the CCL4 control group (respectively, p=0.042; p=0.005; p=0.006; and p=0.001). The higher dose of CGA showed greater ability as an anti-fibrotic through inhibiting the expression of TLR-4. Some research found the expression of TLR-4 has been decreased by treatment of Chlorogenic Acid (CGA).
Conclusion: To sum up, CGA has double roles to repair liver fibrotic tissues. The greater doses of CGA, the stronger inhibition of TLR-4 expression.
Referensi
WHO. 2021. Liver Cirrhosis. WHO online. Home page on-line. Available from Liver cirrhosis, age- standardized death rates (15+), per 100,000 population (who.int); internet access Agustus 1, 2021: 16.00 pm.
United Nation. Good Health and Well-Being. 2020. UN online. Home page on-line. Available from #Envision2030 Goal 3: Good Health and Well-being | United Nations Enable; Internet access Agustus 5, 2021;10.00 am.
Sherwood L. 2014. Human Physiology:From Cells to Systems. 9th Ed. USA:Cengage Learning;593
Guo J, Friedman S. Toll-like receptor 4 signaling in liver injury and hepatic fibrogenesis. J.FT Biomed Central. 2010; 3:21; 1-19.
Yang L, Seki E. Toll-like receptor in liver fibrosis: cellular crosstalk and mechanisms. J. Front. in Physiol. 2012; 3: 1-18.
Seruga M, Tomac I. Electrochemical Properties of Chlorogenic Acids and Determination of Their Content in Coffee Using Differential Pulse Voltametry. Int.J.Electrochem. Sci. 2016; 11:2854- 2876.
Kuhnert N., Said I.H., & Jaiswal R. 2014. Assignment of Regio-and Stereochemistry of Natural Products Using Mass Spectrometry Chlorogenic Acids and Derivatives as a Case Study. J. Stud. Nat. Prod. Chem. 42; 305-339.
PubChem. Compound Chlorogenic Acid . PubChem online. Home page on-line. Available from https://pubchem.ncbi.nlm.nih.gov/compound/chlorogenic_acid# section=KEGG—Phytochemical- Compoun-ds; Internet Access November 22, 2016: 1.14 pm.
Dong L, Han N, Hou N, Li J, Yan Y. Chlorogenic Acid Enhances The Effect of 5- Fluorouracil in Human Hepatocellulear Carcinoma Cells Through The Inhibition of Extracellular Signal-Regulated Kinases. Preclinical Report : Anti-cancer Drugs. 2015; 26(5):540-546.
Pena MPD, Pyrzynska K, Sentkowska A, Skowron MJ. Chlorogenic Acids, Caffeine Content and Antioxidant Properties of Green Coffee Extracts: Influence of Green Coffee Bean Preparation. Eur Food Res Technol J. 2016; 242:1403-1409.
Chemical Book. Chlorogenic Acid. Chemical Book online. Home page on-line. Available from http://www.chemicalbook.com/Chemical Product Property _EN_cb2478906.html. Internet access: November 17, 2016 ; 4.00 pm.
Naibey, R. Pengaruh Pemberian Asam Klorogenat (CGA) Terhadap Fibrosis Hepar Mencit Jantan yang Diinduksi dengan Karbon Tetraklorida (CCL4). Tesis S2. Oktober 2017; 32-43.
Yu, C.,Wang, F., Jin, C., Huang, X., Miller, D.L., Basilico, C., & Mckeehan,W.L. 2003. Role of fibroblast growth factor type 1 and 2 in carbon tetrachloride-induced hepatic injury and fibrogenesis. Am. J. Pathol. 163;1653– 1662.
Wang F.S., Xu R., & Zang Z. 2012. Liver fibrosis: mechanisms of immune-mediated liver injury. Cell. Mol. Imm. J. 9;296-301.
Shi H, Shi A, Dong L, Lu X, Wang Y, Zhao J, Dai F, Guo X. Chlorogenic acid protects against liver fibrosis in vivo and in vitro through inhibition of oxidative stress. Clin. Nut, J. 2015; 35:1366-1373.
Guo J, Friedman S. Toll-like receptor 4 signaling in liver injury and hepatic fibrogenesis. J.FTR Biomed Central. 2010; 3:21; 1-19.
Blouin A, Bolender RP, Weibel ER. Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study. J Cell Biol. 1977;72:441–55. [PMC free article] [PubMed] [Google Scholar]
Bataller R., & Brenner D. 2005. Liver Fibrosis. Clin. Invest. J. 2 (115);209-2118.
Basilico C., Yu C., Huang X., Jin C., Mckeehan W. L., Miller D.L., & Wang F., 2003. Role of fibroblast growth factor type 1 and 2 in carbon tetrachloride-induced hepatic injury and fibrogenesis. Am. J. Pathol. 163;1653– 1662.
Bataille F., Hellerbrand C., Steiling H., Muhlbauer M., Scholmerich J., & Werner S. 2004. Activated hepatic stellate cells express keratinocyte growth factor in chronic liver disease. Am. J. Pathol. 165;1233–1241.
Dockal M., Niiya M., Pollak E.S., Scheiflinger F., Uemura M., Zheng X.W., Wells, R.G., Zheng, X.L., 2006. Increased ADAMTS-13 proteolytic activity in rat hepatic stellate cells upon activation in vitro and in vivo. Thromb. Haemost J. 4;1063–1070.
Kumar H., Kawai T., & Akira S. 2009. Toll-like receptors and innate immunity. J. Biochem. Biophysic. Res.Com. 388; 621-625.
Shi H, Shi A, Dong L, Lu X, Wang Y, Zhao J, Dai F, Guo X. Chlorogenic acid protects against
