The Effect of Trigona Honey as a Therapy for Burns in Rattus Norvegicus
DOI:
https://doi.org/10.33096/37dg8p79Keywords:
Burns, honey, trigona spp, rattus norvegicusAbstract
Introduction: Wound healing is an important physiological process to maintain skin integrity after trauma. Contact burns are damage or loss of tissue caused by heat sources. The growing interest in natural therapies has prompted the exploration of trigona honey due to its antioxidant, anti-inflammatory, and antimicrobial properties. Another study discusses trigona honey on an incised wound, but only a few discuss burns. This study evaluates the effectiveness of Trigona spp honey as a therapy for burns in Rattus norvegicus.
Methods: A quasi-experimental with a non-randomized control group pretest-posttest design method. In this study used 20 subjects, there are Rattus norvegicus of the Sprague Dawley strain weighing 100-250 grams and aged 2-3 months, divided into 5 subjects for 4 groups. This study assesses wound size and use one-way ANOVA as the analysis technique.
Result: There is significant wound healing initially in all groups before intervention, average wound area is 3.5 cm. Then treatment was given for 15 days and it was seen that the group with the best wound healing was treatment on group II (Trigona honey 2 g) with an average wound area of 0.42 cm, followed by treatment group I (Trigona honey 1 g) is 0.72 cm, followed by the positive control group (silver sulfadizine 0.5 g) is 0.86 cm and then on negative control group (aquadest) is 1.74 cm. In the one-way ANOVA test, the P value (Between Groups) was 0.000 (<5%).
Conclusion: Trigona honey is effective as a therapy for burns in rattus norvegicus.
References
1. Kaddoura, Sittah.,A,Ibrahim,A,et al. Burn Injury of Pathophysiology and Therapeutic Modalities in Major Burns. Annals of Burns and Fire Disasters. 2017: 95-102.
https://pmc.ncbi.nlm.nih.gov/articles/PMC5627559/pdf/Ann-Burns-and-Fire-Disasters-30-95.pdf. Accessed June 30, 2024.
2. Nielson,B,C., Nicholas C.,Moncure,M,et al. Burns: Pathophysiology of Systemic Complication and Current Management. Journal of Burn Care & research. 2017: 469-480.
https://pmc.ncbi.nlm.nih.gov/articles/PMC5214064/pdf/bcr-38-e469.pdf. Accessed June 30, 2024.
3. Zeline,M.A.,dkk. Kajian Literatur Potensi Tanaman-Tanaman Obat Untuk Mengatasi Luka Bakar. 12th Proc. Mul. Pharm. Conf. 2020. https://prosiding.ff.unmul.ac.id/index.php/mpc/article/view/191. Accessed June 30, 2024
4. Sari, DP.,dkk. Lidah Buaya (Aloe vera) Sebagai Obat Herbal Penyembuahan Luka. Journal of Natural Sciences, Vol. 3, No. 3, November 2022: 128-136. Accessed June 30, 2024
5. Julianto, dkk. Perawatan Luka Bakar dengan Tanaman Obat. 2019. Yogyakarta: ANDI
6. Ajibola, A., Chamunorwa, J.P. and Erlwanger, K.H. Nutraceutical values of natural honey and its contribution to human health and wealth. Nutrition & metabolism. 2012;9(61):1-12. https://pmc.ncbi.nlm.nih.gov/articles/PMC3583289/pdf/1743-7075-9-61.pdf. Accessed July 02, 2024.
7. Al-Waili, N.S. Effects of daily consumption of honey solution on hematological indices and blood levels of minerals and enzymes in normal individuals. Journal of medicinal food. 2003;6(2):135-40. https://pubmed.ncbi.nlm.nih.gov/12935325/. Accessed July 02, 2024.
8. Fuenmayor,C.A., Dominguez C.M., Moreno,AC. Honey of Colombian Stingless Bees: Nutritional Characteristics and Physicochemical Quality Indicators. In : Pot Honey, A Legacy of stingless bees.1st ed. Springer.2013. https://www.researchgate.net/publication/235946302_Honey_of_Colombian_Stingless_Bees_Nutritional_Cha racteristics_and_Physicochemical_Quality_Indicators. Accessed July 02, 2024
9. Hossen,S., Daim,M.A.,Ali, Y. Beneficial roles of honey polyphenols against some human degenerative diseases: A review. Elsevier. 2017;69 :1194-1205. https://apitherapy.com/wp-content/uploads/2021/06/Beneficial_roles_of_honey_polyphenols_against-degenerative-diseases.pdf. Accessed July 05, 2024
10. Ariefdjohan, M.W. et al. Acute and chronic effects of honey and its carbohydrate constituents on calcium absorption in rats. Journal of agricultural and food chemistry 2008; 56(8):2649-2654. https://pubmed.ncbi.nlm.nih.gov/18361495/. Accessed July 06, 2024
11. Al-Qur’an
12. Mustafa,MZ, et al. Anti-inflammatory Properties of Stingless Bee Honey May Reduce the Severity of Pulmonary Manifestations in COVID-19 Infections. Malays J Med Sci. 2020;27(2):165–169. https://pmc.ncbi.nlm.nih.gov/articles/PMC7409568/pdf/17mjms27022020_le.pdf. Accessed July 06, 2024
13. Ranneh,Y,et.al. Stingless bee honey protects against lipopolysaccharide induced-chronic subclinical systemic inflammation and oxidative stress by modulating Nrf2, NF-κB and p38 MAPK. Nutrition & Metabolism. 2019; 16:1-17. https://pmc.ncbi.nlm.nih.gov/articles/PMC6391794/pdf/12986_2019_Article_341.pdf. Accessed July 06, 2024
14. Arifin, W.N. and Zahiruddin, W.M. Sample size calculation in animal studies using resource equation approach. The Malaysian journal of medical sciences. 2017;24(5): 101-105.
https://pmc.ncbi.nlm.nih.gov/articles/PMC5772820/pdf/11mjms24052017_bc.pdf . Accessed July 03, 2024.
15. Rahmawati, Marlina, Nurhidayah. Efektivitas Penggunaan Madu dalam Proses Penyembuhan Pada Luka Bakar Derajat II: Literatur Review. Idea Nursing Journal. 2020;7 (2): 2580-2445.
https://jurnal.umsb.ac.id/index.php/menaramedika/article/view/5955. Accessed July 05, 2024.
16. Wulandari. Kualitas Madu (Keasaman, Kadar Air, dan Kadar Gula Pereduksi) Berdasarkan Perbedaan Suhu Penyimpanan. Jurnal Kimia Riset. 2017; 2 (1): 16-22. https://e-journal.unair.ac.id/JKR/article/view/3768/3135.
Accessed December 05, 2024.
17. Kalangi, S.J. Khasiat Madu pada Penyembuhan Luka. Jurnal Biomedik. 2012; 4(3):163-166. https://ejournal.unsrat.ac.id/index.php/biomedik/article/view/796. Accessed December 23, 2024.
18. Hendy,Lister,N.E. Tingkat Efektivitas Penyembuhan Luka Bakar Derajat II a dengan Pemberian Madu dan Pemberian Salep Nebacetin Pada Tikus Putih. Jurnal Kedokteran dan Kesehatan. 2019; 15(2):130.
